Transcriptome profiling of antiviral immune and dietary fatty acid dependent responses of Atlantic salmon macrophage-like cells

Table 4 Comparison between the microarray and qPCR results of a subset of 9 diet-responsive transcripts identified by Rank Product (RP)

Microarray Probe ID	Name	FO5/FO7, Microarray fold-change PBS@ 24 HPS¹	FO5/FO7, Microarray fold-change pIC @ 24 HPS¹	FO5/FO7, qPCR fold-changePBS @ 6 HPS	FO5/FO7, qPCR fold-change pIC @ 6 HPS	FO5/FO7, qPCR fold-change PBS @ 24 HPS	FO5/FO7, qPCR fold-change pIC @ 24 HPS	qPCR, p value PBS²	qPCR, p value pIC²
C148R063	Fc receptor-like protein 2 (fcr2)	3.63	3.72	1.13	1.03	1.24	1.21	0.42	0.53
C108R146	fatty acid-binding protein, adipocyte (fabp4)	2.72	2.98	4.99	3.91	5.29*	4.39*	0.0012	0.003
C126R012	FAD-linked sulfhydryl oxidase ALR-like (fadox)	2.21	–	1.15	1.09	1.54	1.07	0.082	0.22
C001R074	lathosterol oxidase (sc5d)	–	1.96	1.22	1.06	1.30	1.36	0.15	0.36
C146R053	legumain (lgmn)	–	1.83	1.72	1.83*	1.32	1.13	0.024³	0.022
C096R058	tropomodulin-4-like (tmod4)	–	0.43	0.38	0.49	0.73	0.31	0.039³	0.064
C153R016	phospholipase d4 (pld4)	0.43	0.47	1.19	1.17	0.58	0.62	0.46	0.56
C027R162	MHC class I antigen	0.29	0.32	0.92	0.95	1.95	1.26	0.44	0.72
C164R003	proteasome subunit beta type-8 (psmb8)	0.28	0.23	0.0024	0.0016	0.0013*	0.00088*	0.011	0.016

¹The fold changes between PBS- or pIC-matched groups of FO5 and FO7 at the same time (FO5/FO7). A dash (−) represents no differential expression between groups for a given comparison in microarray analyses
²The p values of qPCR results as implemented by two-way ANOVA between PBS- and pIC-matched groups of dietary treatments
³The significant p values were observed for PBS-matched groups of dietary treatments, but no significant difference was found by Sidak multiple comparisons post hoc test
*Significant difference (p < 0.05) between pIC- and PBS-matched groups of dietary treatments in qPCR assay

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