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Table 4 Comparison between the microarray and qPCR results of a subset of 9 diet-responsive transcripts identified by Rank Product (RP)

From: Transcriptome profiling of antiviral immune and dietary fatty acid dependent responses of Atlantic salmon macrophage-like cells

Microarray Probe ID Name FO5/FO7, Microarray
fold-change PBS@ 24 HPS1
FO5/FO7, Microarray
fold-change pIC @ 24 HPS1
FO5/FO7, qPCR fold-changePBS @ 6 HPS FO5/FO7, qPCR fold-change pIC @ 6 HPS FO5/FO7, qPCR fold-change PBS @ 24 HPS FO5/FO7, qPCR fold-change pIC @ 24 HPS qPCR, p value PBS2 qPCR, p value pIC2
C148R063 Fc receptor-like protein 2 (fcr2) 3.63 3.72 1.13 1.03 1.24 1.21 0.42 0.53
C108R146 fatty acid-binding protein, adipocyte (fabp4) 2.72 2.98 4.99 3.91 5.29* 4.39* 0.0012 0.003
C126R012 FAD-linked sulfhydryl oxidase ALR-like (fadox) 2.21 1.15 1.09 1.54 1.07 0.082 0.22
C001R074 lathosterol oxidase (sc5d) 1.96 1.22 1.06 1.30 1.36 0.15 0.36
C146R053 legumain (lgmn) 1.83 1.72 1.83* 1.32 1.13 0.0243 0.022
C096R058 tropomodulin-4-like (tmod4) 0.43 0.38 0.49 0.73 0.31 0.0393 0.064
C153R016 phospholipase d4 (pld4) 0.43 0.47 1.19 1.17 0.58 0.62 0.46 0.56
C027R162 MHC class I antigen 0.29 0.32 0.92 0.95 1.95 1.26 0.44 0.72
C164R003 proteasome subunit beta type-8 (psmb8) 0.28 0.23 0.0024 0.0016 0.0013* 0.00088* 0.011 0.016
  1. 1The fold changes between PBS- or pIC-matched groups of FO5 and FO7 at the same time (FO5/FO7). A dash (−) represents no differential expression between groups for a given comparison in microarray analyses
  2. 2The p values of qPCR results as implemented by two-way ANOVA between PBS- and pIC-matched groups of dietary treatments
  3. 3The significant p values were observed for PBS-matched groups of dietary treatments, but no significant difference was found by Sidak multiple comparisons post hoc test
  4. *Significant difference (p < 0.05) between pIC- and PBS-matched groups of dietary treatments in qPCR assay