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Table 3 Primers

From: Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84

Primer

Sequence (5′- 3′)

Bmh1-kanMX- Fw

GCAAGTGAGAAGAAAAAGCAAGTTAAAGATAAACTAAAGATAAAACAGCTGAAGCTTCGTACGC

Bmh1-kanMX-Rv

AGATTTATCAGAATACTTACTTTGGTGCTTCACCTTCGGCGGCAGCGCATAGGCCACTAGTGGATCTG

Bmh2-kanMX-Fw

GAAAAATTATCAAATCAACAAAAAGTACCCGTTACAACAAAAAAACAGCTGAAGCTTCGTACGC

Bmh2-kanMX-Rv

GCAAGAAAACTGGAGTGGTAAATCTTCATTTCCCCTTGTATTTCTGCATAGGCCACTAGTGGATCTG

PHO84-qPCR-Fw

ACAACCTTG TTGATCCCAG AA

PHO84-qPCR-Rv

TGCTTCATGTTGAAGTTGAGATG

SPL2-qPCR-Fw

CCGAGGAGATCGCTTCTCTA

SPL2-qPCR-Rv

ACGCTGCGCTCTACTTGAAT

ACT1-qPCR-Fw

CTGCCGGTATTGACCAAACT

ACT1-qPCR-Rv

CGGTGATTTCCTTTTGCATT

PHO80-kanMX-Fw

AAGCTATCATAAGACGAGGATATCCTTTGGAGACTCATAGAAATCCAGCTGAAGCTTCGTACGC

PHO80-kanMX-Rv

TTTTGCTCAATCATGATTGCTTTCATAATACCCCACGAAAAATCACCGCGGCCGCATAGGCCAC

SPL2-GFP-Fw2

ATTGACGAAGACATATTCGAAGATTCGTCTGACGAAGAACAATCACGTACGCTGCAGGTCGAC

SPL2-GFP-Rv

GTCAATGCATATGTAACAGTACAGAGGTAGAAGGTATGTGTATCGATGAATTCGAGCTCG

P-pho84-Fw

AAA GAGCTC AATCAGTATT ACGCACGTTGGT

P-pho84-Rv

AAA ACTAGT CATTTGGATTGTATTCGTGGAGT

T-pho84-Fw

AAA GGATCC TAAAAGCCT CAAAGATGCA CTAA

T-pho84-Rv

AAA GAATTC CTGTCCCACAGGTGCCATTG

P-cyc1-Fw

AAA GAGCTC GTTCATTTGG CGAGCGTTGG

P-cyc1-Rv

AAA ACTAGT CATTATTAATTTAGTGTGTGTATTTG

T-cyc1-Fw

AAA GGATCC TAA ACAGGCCCCT TTTCCTTTGTC

T-cyc1-Rv

AAA GAATTC ATGTTACATGCGTACACGCGT

P-spl2-Fw

AAA GAGCTC TTTACACTGGGATATTACAAGAC C

P-spl2-Rv2

AAA ACTAGT CATCTGTCCAATTTGCCCCTG

T-spl2-Fw

AAA GGATCC TGATTGCATCTCTTAATCGTTACAC

T-spl2-Rv

AAA GAATTC AAAGGGCCAGCGAATGCGCG

GFP-Fw

AAA ACTAGT ATGTCTAAAGGTGAAGAATTATTCA

GFP-Rv

AAA GGATCC TTTGTACAATTCATCCATACCATA

PHO4-Fw

GG GAATTC GTCTCTGTCTAATGCGGTCAC

PHO4-Rv

GG GGATCC GTTCTCTCAAATCTTCCAACTGATC

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BMC Genomics

ISSN: 1471-2164

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