Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84

Table 4 Effect of bmh1 and bmh2 deletion on RNA levels of PHO84 and SPL2 (SAGE-tag sequencing)

	RNA level (reads per million) (± SD)
Gene	BY4741		bmh1Δ		bmh2Δ
Gene	50 mM (n = 4)	0 mM (n = 4)	50 mM (n = 3)	0 mM (n = 3)	50 mM (n = 3)	0 mM (n = 3)
PHO84	50 ± 27	516 ± 214	2 ± 1^a	243 ± 140	6 ± 0.5^c	235 ± 16
SPL2	31 ± 5	224 ± 69	3 ± 0^b	95 ± 33	7 ± 1^d	163 ± 26

^aStudent’s t-test indicated a significant difference between bmh1Δ and BY4741 at 50 mM KCl (P = 0.03)
^bStudent’s t-test indicated a significant difference between bmh1Δ and BY4741 at 50 mM KCl (P = 0.0002)
^cStudent’s t-test indicated a significant difference between bmh2Δ and BY4741 at 50 mM KCl (P = 0.04)
^dStudent’s t-test indicated a significant difference between bmh2Δ and BY4741 at 50 mM KCl (P = 0.004)

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