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Table 4 Effect of bmh1 and bmh2 deletion on RNA levels of PHO84 and SPL2 (SAGE-tag sequencing)

From: Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84

  RNA level (reads per million) (± SD)
Gene BY4741 bmh1Δ bmh2Δ
50 mM
(n = 4)
0 mM
(n = 4)
50 mM
(n = 3)
0 mM
(n = 3)
50 mM
(n = 3)
0 mM
(n = 3)
PHO84 50 ± 27 516 ± 214 2 ± 1a 243 ± 140 6 ± 0.5c 235 ± 16
SPL2 31 ± 5 224 ± 69 3 ± 0b 95 ± 33 7 ± 1d 163 ± 26
  1. aStudent’s t-test indicated a significant difference between bmh1Δ and BY4741 at 50 mM KCl (P = 0.03)
  2. bStudent’s t-test indicated a significant difference between bmh1Δ and BY4741 at 50 mM KCl (P = 0.0002)
  3. cStudent’s t-test indicated a significant difference between bmh2Δ and BY4741 at 50 mM KCl (P = 0.04)
  4. dStudent’s t-test indicated a significant difference between bmh2Δ and BY4741 at 50 mM KCl (P = 0.004)