Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84

Table 6 Activity of the PHO84, SPL2 and CYC1 promoter determined by flow cytometry using GFP reporters

	GFP fluorescence (arbitrary units) (± SD; n = 3)
Strain	50 mM	0 mM
Experiments PHO84
BY4741 P_PHO84– GFP–T_PHO84	30 ± 1	58 ± 2
BY4741 P_CYC1 – GFP–T_CYC1	198 ± 29	179 ± 8
bmh1Δ P_PHO84– GFP–T_PHO84	13 ± 2^a	37 ± 3
bmh1Δ P_CYC1 – GFP–T_CYC1	204 ± 12	146 ± 54
Experiments SPL2
BY4741 P_SPL2– GFP–T_SPl2	3.7 ± 1.6	7.1 ± 2.8
BY4741 P_CYC1 – GFP–T_CYC1	204 ± 12	219 ± 30
bmh1Δ P_SPL2– GFP–T_SPL2	0.8 ± 0.3^b	2.7 ± 0.5
bmh1Δ P_CYC1 – GFP–T_CYC1	131 ± 5	139 ± 24

^aStudent’s t-test indicated a significant difference between bmh1Δ and BY4741 at 50 mM KCl (P < 0.001)
^bStudent’s t-test indicated a significant difference between bmh1Δ and BY4741 at 50 mM KCl (P = 0.03)

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