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Table 6 Activity of the PHO84, SPL2 and CYC1 promoter determined by flow cytometry using GFP reporters

From: Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84

  GFP fluorescence (arbitrary units) (± SD; n = 3)
Strain 50 mM 0 mM
Experiments PHO84
 BY4741 PPHO84– GFP–TPHO84 30 ± 1 58 ± 2
 BY4741 PCYC1 – GFP–TCYC1 198 ± 29 179 ± 8
 bmh1Δ PPHO84– GFP–TPHO84 13 ± 2a 37 ± 3
 bmh1Δ PCYC1 – GFP–TCYC1 204 ± 12 146 ± 54
Experiments SPL2
 BY4741 PSPL2– GFP–TSPl2 3.7 ± 1.6 7.1 ± 2.8
 BY4741 PCYC1 – GFP–TCYC1 204 ± 12 219 ± 30
 bmh1Δ PSPL2– GFP–TSPL2 0.8 ± 0.3b 2.7 ± 0.5
 bmh1Δ PCYC1 – GFP–TCYC1 131 ± 5 139 ± 24
  1. aStudent’s t-test indicated a significant difference between bmh1Δ and BY4741 at 50 mM KCl (P < 0.001)
  2. bStudent’s t-test indicated a significant difference between bmh1Δ and BY4741 at 50 mM KCl (P = 0.03)