From: Impaired response of the bronchial epithelium to inflammation characterizes severe equine asthma
Gene symbol | Positive regulation of immune system process | Immune response | Inflammatory response | Positive regulation of response to external stimulus | Secretion | Positive regulation of apoptotic signalling pathway | Neutrophil chemotaxis | Positive regulation of blood coagulation | Association with asthma | Protein function (NCBI or Uniprot; Homo sapiens) |
---|---|---|---|---|---|---|---|---|---|---|
S100A9 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | Promotes inflammation with and without TLR4 interactions in a mouse model [38] | Regulation of cellular processes such as cell cycle and differentiation. Antifungal and antibacterial activity |
THBS1 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | × | ✓ | Central in a network linked to pulmonary response to oxidative stress in asthma [39] | Cell-to-cell and cell-to-matrix interactions |
TLR4 | ✓ | ✓ | ✓ | ✓ | ✓ | × | × | ✓ | Linked to allergic asthma in a mouse model [41] | Pathogen recognition and innate immunity |
CXCL8 | ✓ | ✓ | ✓ | ✓ | × | × | ✓ | × | Biomarker of neutrophilic asthma in adult [20] | Chemoattractant and angiogenic factor |
C5AR1 | ✓ | ✓ | ✓ | ✓ | × | × | ✓ | × | Linked to regulation of airway remodelling [42] | Receptor for C5a, ligand with chemotactic and inflammatory activity |
MMP9 | ✓ | ✓ | ✓ | × | ✓ | ✓ | × | × | Increased in severe asthma, involved in Th2 response and EMT in human bronchial epithelial cells [43, 44] | Matrix metalloproteinase, breakdown of extracellular matrix |
NLRP12 | ✓ | ✓ | ✓ | ✓ | ✓ | × | × | × | Increased expression in asthmatics on high-fat diet. No observed effect in allergic airway disease models [45,46,47] | Anti-inflammatory molecule |
TREM1 | ✓ | ✓ | × | × | ✓ | × | ✓ | × | Correlates with clinical stage and neutrophils in human asthmatics [48] | Amplifies neutrophil and monocyte inflammatory response |
PLEK | ✓ | × | × | × | ✓ | × | × | ✓ | NAa | EMT in bronchial epithelial cells [47] |
LGALS3 | ✓ | ✓ | × | × | × | × | ✓ | × | Key role in inflammation and airway remodelling in murine models of allergic asthma [95,96,97]. Decreased in sputum of neutrophilic asthmatics compared to mixed neutrophilic and eosinophilic asthmatics [98] | Involved in apoptosis, innate immunity, cell adhesion and T-cell regulation Antimicrobial activity |
OSM | × | ✓ | × | ✓ | × | ✓ | × | × | Part of IL6 family of cytokines, OSM specifically is increased in asthmatics and correlates with irreversible airway obstruction [99]. OSM was associated with asthma but not atopy in children [100] | Inhibition of proliferation, regulation of production of other cytokines |
PTGS2 | × | × | ✓ | ✓ | ✓ | × | × | × | Polymorphism in the gene associated with asthma and atopy in children [101] | Prostaglandin biosynthesis |
ORM1 | ✓ | × | ✓ | × | ✓ | × | × | × | NA | Acute-phase protein, potential immunosuppressive activity |
IL1RN | × | ✓ | ✓ | × | ✓ | × | × | × | Sputum IL1RN to IL-1β ratio decreased in neutrophilic asthmatics compared to mixed neutrophilic and eosinophilic asthmatics [98] | EMT in bronchial epithelial cells [47] Modulates IL1-mediated inflammation and inhibits activity of IL1A & B |
CSF3R | ✓ | × | × | × | × | × | ✓ | × | NA | Controls granulocyte production and activity. |
CXCR2 | × | × | ✓ | × | × | × | ✓ | × | Expression increased in sputum of patient with non-eosinophilic asthma [21]. Controversial role in asthma. CXCR2 antagonists alone do not improve clinical signs of asthma but reduce neutrophil number [21]. Combination of CXCR1 and CXCR2 antagonists promising for therapy [102] | IL8 receptor, neutrophil migration |
CAMP | × | ✓ | ✓ | × | × | × | × | × | NA | Response to pathogens, Antibacterial, regulation of cell chemotaxis and inflammation. |
PTX3 | × | ✓ | ✓ | × | × | × | × | × | Expression increased in bronchial tissues of asthmatics and highly expressed in smooth muscle cells [103] | Up-regulated in response to inflammation in epithelial cells. Role in angiogenesis and tissue remodelling. |
MRGPRX2 | × | ✓ | × | × | ✓ | × | × | × | NA | IgE independent activation of mast-cells, leading to inflammation and smooth muscle cell contraction [104]. Mast cell-specific receptor, mediates allergic reactions. |
AQP9 | × | ✓ | × | × | ✓ | × | × | × | NA | Neutrophil regulation [105]; membrane channel |
CLEC4E | ✓ | ✓ | × | × | × | × | × | × | NA | Inflammation |
LILRB3 | ✓ | ✓ | × | × | × | × | × | × | NA | Inhibits immune response |
CD300LB | ✓ | ✓ | × | × | × | × | × | × | NA | Expressed by granulocytes [106]. Activates immune receptor |
Specific to one gene set | ||||||||||
TNFRSF12A | × | × | × | × | × | ✓ | × | × | NA | May be involved in angiogenesis, proliferation and cellular adhesion |
SFN | × | × | × | × | × | ✓ | × | × | NA | Regulation of protein kinase C [107] and epithelial cell growth through Akt/mTOR pathway when bound to KRT17 [108] |
PLAUR | × | × | × | × | × | ✓ | × | × | Increased expression in vitro in bronchial epithelium from asthmatics [109] | Impaired wound repair process [109]. May be involved in plasminogen activation and ECM degradation |
OLR1 | × | × | ✓ | × | × | × | × | × | NA | Degradation of oxidized low-density lipoprotein |
CCRL2 | × | × | ✓ | × | × | × | × | × | Expressed in lung epithelial cells and up-regulated following LPS exposure. Potential role in initiation of allergic inflammation [110] | Unknown function, up-regulated in activated neutrophils |
SERPINE2 | × | × | × | × | ✓ | × | × | × | Urokinase inhibitor [111], overexpression in asthma and down-regulation correlated with improved FEV1 [112] | Serine protease inhibition |
SRGN | × | × | × | × | ✓ | × | × | × | NA | Hematopoietic cell granule proteoglycan |
TUBA4A | × | × | × | × | ✓ | × | × | × | NA | Component of microtubules |
FCAR | × | ✓ | × | × | × | × | × | × | NA | Surface receptor of myeloid cells, regulation of immune defense processes |
PADI4 | × | ✓ | × | × | × | × | × | × | NA | Required for NET formation [79] in equine asthma [78], conversion of arginine to citrulline |
APOBEC3B | × | ✓ | × | × | × | × | × | × | NA | Up-regulated by PKC and increased likelihood of mutations [113], RNA editing |
TREML4 | ✓ | × | × | × | × | × | × | × | NA | Immune response |
CD300LD | ✓ | × | × | × | × | × | × | × | NA | Immune response |
CD300A | ✓ | × | × | × | × | × | × | × | Co-aggregation of CD300A with CCR3 decreased clinical signs of asthma in a mouse model [114] | Regulation of mast cell response [115]. Surface protein on leukocytes involved in immune response |
SLC46A2 | ✓ | × | × | × | × | × | × | × | NA | Potential transporter function |
SAMSN1 | ✓ | × | × | × | × | × | × | × | NA | Cell proliferation, spreading and regulation of polarization |
TREML2 | ✓ | × | × | × | × | × | × | × | Associated with asthma but not atopy in children [100] | Surface receptor, role in innate and adaptive immune response |