Table 3 Changes in mRNA levels in human CRC cell lines treated by different chemotherapeutic drugs and/or by Lovastatin. After 24 h of treatment by different chemotherapeutic drugs (Oxaliplatin (10 μM), 5-Fluorouracile (10 μM), Camptothecin (0.1 μM) in combination or not with Lovastatin (5 μM), total RNA was extracted and submitted to reverse transcription. Real-time PCR for different genes was performed. Three independent experiments were analyzed. Fold-change was indicated when the p-value was significant Student’s-t test)

CHECK2

HCT-116

NC

0.69*

NC

NC

0.56***

0.44**

NC

HT-29

2.74*

2.43

1.40*

0.51*

2.60*

1.84*

2.33**

CYP51A1

HCT-116

NC

0.44*

0.31*

NC

0.56**

0.22**

(0.64)

CYP11A1

HCT-116

NC

NC

NC

NC

0.53*

0.78**

NC

BCL2

HCT-116

0.23***

0.46*

0.52*

NC

0.14***

0.17***

0.61**

NME1

HCT-116

2.72***

NC

NC

NC

0.60**

0.30**

0.52*

HT-29

1.81*

NC

NC

0.52*

NC

(0.61)

NC

ITGA2

HCT-116

1.77**

NC

NC

NC

0.68*

0.35*

NC

HT-29

NC

NC

(0.41)

0.38*

0.50**

(0.51)

0.47**

IL8

HCT-116

3.35***

NC

NC

NC

(3.87)

3.37*

(3.01)

HT-29

2.92*

4.74*

2.33*

0.64***

3.24*

3.81*

3.52***

NOS3

HCT-116

1.87**

1.63***

NC

NC

NC

2.13*

NC

HT-29

NC

3.42**

NC

0.56**

NC

1.97*

(2.16)

DHCR7

HCT-116

NC

NC

0.48*

NC

NC

NC

0.49**

HT-29

NC

NC

NC

NC

1.50*

2.29***

NC

HMGCS1

HCT-116

0.82*

0.46**

0.23***

NC

NC

NC

0.32*

HT-29

NC

0.37**

0.42*

NC

NC

NC

NC

VEGFA

HT-29

NC

NC

NC

0.43**

NC

0.54*

NC