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Table 3 Changes in mRNA levels in human CRC cell lines treated by different chemotherapeutic drugs and/or by Lovastatin. After 24 h of treatment by different chemotherapeutic drugs (Oxaliplatin (10 μM), 5-Fluorouracile (10 μM), Camptothecin (0.1 μM) in combination or not with Lovastatin (5 μM), total RNA was extracted and submitted to reverse transcription. Real-time PCR for different genes was performed. Three independent experiments were analyzed. Fold-change was indicated when the p-value was significant Student’s-t test)

From: A transcriptome-based protein network that identifies new therapeutic targets in colorectal cancer

Gene Symbol Fold-change CRC vs. NT CRC line Oxa 5-FU Cpt Lova Oxa + Lova 5-FU + Lova Cpt + Lova
CHECK2 2.08*** HCT-116 NC 0.69* NC NC 0.56*** 0.44** NC
HT-29 2.74* 2.43 1.40* 0.51* 2.60* 1.84* 2.33**
CYP51A1 2.80*** HCT-116 NC 0.44* 0.31* NC 0.56** 0.22** (0.64)
CYP11A1 0.32** HCT-116 NC NC NC NC 0.53* 0.78** NC
BCL2 0.31*** HCT-116 0.23*** 0.46* 0.52* NC 0.14*** 0.17*** 0.61**
NME1 4.6*** HCT-116 2.72*** NC NC NC 0.60** 0.30** 0.52*
HT-29 1.81* NC NC 0.52* NC (0.61) NC
ITGA2 3.8*** HCT-116 1.77** NC NC NC 0.68* 0.35* NC
HT-29 NC NC (0.41) 0.38* 0.50** (0.51) 0.47**
IL8 8.1** HCT-116 3.35*** NC NC NC (3.87) 3.37* (3.01)
HT-29 2.92* 4.74* 2.33* 0.64*** 3.24* 3.81* 3.52***
NOS3 2.3*** HCT-116 1.87** 1.63*** NC NC NC 2.13* NC
HT-29 NC 3.42** NC 0.56** NC 1.97* (2.16)
DHCR7 3.6*** HCT-116 NC NC 0.48* NC NC NC 0.49**
HT-29 NC NC NC NC 1.50* 2.29*** NC
HMGCS1 2.46*** HCT-116 0.82* 0.46** 0.23*** NC NC NC 0.32*
HT-29 NC 0.37** 0.42* NC NC NC NC
VEGFA 2.04*** HT-29 NC NC NC 0.43** NC 0.54* NC
  1. Abbreviations: Oxa oxaliplatin, 5-FU 5-Fluorouracile, Cpt Camptothecin, Lova Lovastatin
  2. NC no change; * p < 0.05; ** p < 0.01; *** p < 0.001
  3. Numbers in parentheses were not significant on three independent experiments, although a tendency to variation was recorded in two out of three experiments