Fig. 2

Experimental characterization of EXBs. a Error rate between single-end EXB sequence and best decoder matrix match. One million reads from each bulk sample type and input amount are pooled. The mean mismatch rate between the experimentally-derived EXB sequence and the best decoder matrix match is plotted as a percentage of all sequenced EXB bases against the base position along the EXB structure. b GC bias of single-end EXBs. One million reads from each library were pooled. The mean GC content for both read 1 and 2 of the barcode containing region (18 bp total) is calculated for each observed single-end EXB. An ideal GC-content distribution corresponding to a uniform distribution of all single-end EXBs is shown as dots. t-test results: p = 1 (ideal versus read 1), and p = 1 (ideal versus read 2), indicating non-significant differences between distributions