Fig. 3

Transcriptome quantification with EXBs. a EXB processing workflow. Reads with the same paired-end barcode subunit sequences are group together into read groups. These read groups are then collapsed into consensus reads for downstream alignment and quantification. b EXB duplication rate. The number of aligning reads per paired-end EXB group is measured as a proportion of total reads. c Fold-change levels compared between previously determined MAQC RT-qPCR data and EXB-based expression levels for 10 ng input cDNA