Table 1 Strains analyzed in the study, experimental test for resistance and mutations in pyrazinamidase
Resistance profile | Mutations in pncA/PZAse | |||||
|---|---|---|---|---|---|---|
Isolate | BACTEC 460 TB | BACTEC 960 TB-MGIT | Res PZA | Gene | Protein | Promoter |
CSV4519 | R | – | 1 | – | – | – |
CSV4644 | R | – | 1 | T152C | H51R | – |
CSV5769 | R | R | 1 | C232A | G78C | – |
CSV10399 | R | R | 1 | A280G | F94 L | – |
CSV11678 | R | – | 1 | G185A | P62L | – |
LE486 | R | – | 1 | T35G | D12A | – |
LE492 | – | R | 1 | T35G | D12A | – |
LN180 | – | R | 1 | A392C | V131G | – |
LN2358 | – | R | 1 | A545C | L182 W | – |
LN3756 | R | – | 1 | T403G | T135P | – |
MDRDM260 | – | R | 1 | T403G | T135P | – |
MDRDM627 | – | R | 1 | – | – | – |
MDRDM1098 | R | R | 1 | C145T | D49N | – |
MDRMA2491 | R | R | 1 | T35C | D12G | – |
ME1473 | R | – | 1 | A100C | Y34D | – |
TBDM425 | R | – | 1 | C145T | D49N | – |
TBV5000 | R | – | 1 | – | – | – |
TBV5362 | R | – | 1 | – | – | – |
TBV5365 | R | – | 1 | – | – | – |
SLM036 | R | R | 1 | A190C | Y64D | – |
SLM040 | R | – | 1 | T170C | H57R | – |
SLM056 | R | – | 1 | – | – | – |
SLM060 | R | – | 1 | – | – | T-11C |
SLM063 | R | – | 1 | A416G | V139A | – |
SLM088 | R | – | 1 | – | – | – |
SLM100 | R | – | 1 | – | – | T-11C |
CSV383 | S | – | 0 | A40C | C14G | |
CSV3611 | S | – | 0 | – | – | |
CSV9577 | S | – | 0 | – | – | |
LE13 | – | S | 0 | T478C | T160A | |
LE63 | – | S | 0 | – | – | |
LE76 | – | S | 0 | – | – | |
LE79 | – | S | 0 | – | – | |
LE103 | S | S | 0 | – | – | |
LE371 | – | S | 0 | – | – | |
LE410 | – | S | 0 | – | – | |
LN55 | – | S | 0 | A40C | C14G | |
LN317 | – | S | 0 | – | – | |
LN763 | – | S | 0 | – | – | |
LN2978 | – | S | 0 | – | – | |
LN3584 | – | S | 0 | – | – | |
LN3588 | – | S | 0 | – | – | |
LN3589 | – | S | 0 | – | – | |
LN3668 | – | S | 0 | – | – | |
LN3672 | – | S | 0 | – | – | |
LN3695 | – | S | 0 | – | – | |
LN1100 | – | S | 0 | – | – | |
LN1856 | S | S | 0 | T143G | K48 T | |
LN2900 | – | S | 0 | – | – | |
MDRDM827 | – | S | 0 | – | – | |
MDRMA203 | – | S | 0 | – | – | |
MDRMA701 | – | S | 0 | – | – | |
MDRMA863 | – | S | 0 | – | – | |
MDRMA1565 | – | S | 0 | – | – | |
MDRMA2019 | – | S | 0 | – | – | |
MDRMA2082 | – | S | 0 | – | – | |
MDRMA2260 | – | S | 0 | – | – | |
MDRMA2441 | – | S | 0 | – | – | |
TBDM1506 | – | S | 0 | – | – | |
TBDM2189 | – | S | 0 | – | – | |
TBDM2444 | – | S | 0 | – | – | |
TBDM2487 | – | S | 0 | – | – | |
TBDM2489 | – | S | 0 | – | – | |
TBDM2699 | – | S | 0 | – | – | |
TBDM2717 | – | S | 0 | – | – | |
TBV4766 | S | – | 0 | – | – | |
TBV4768 | S | – | 0 | – | – | |
TBV4952 | S | – | 0 | – | – | |