Single-virion sequencing of lamivudine-treated HBV populations reveal population evolution dynamics and demographic history

Table 1 Patient sample nomenclature and viral copy number

Sample ID	Patient	Date	Viral Copies/ul	Single-virion Sequences	Nucleotide Diversity ∏
1.1	1	15th Nov 94	378,500	1717^†, 1635*	0.0012/base
1.2	1	3rd Jul 99	52,270	3331^†, 2514*	0.0024/base
2.1	2	22nd May 95	239,750	391^†, 1330*	0.0032/base
2.2	2	29th Aug 97	44,687	3747^†, 2504*	0.0013/base
7.1	7	19th May 95	69,180	2647^†	0.0022/base
7.2	7	11th Apr 00	48,083	789^†	0.0016/base
11.1	11	19th Jun 95	208,275	2754^†	0.0211/base
11.2	11	3rd Nov 98	466,200	248^†	0.0052/base

^†-total number of single-virion sequences obtained from a BAsE-Seq library. *-total number of single-virion sequences obtained from a PacBio library. Nucleotide diversity Π, the arithmetic mean between all pairwise differences between viral sequences within each viral population, were calculated from BAsE-Seq single-virion sequences for the entire amplified genomic sequence of 3175 bases (3215 minus the 40 bases that were not amplified)

ISSN: 1471-2164