Sample ID

Patient

Date

Viral Copies/ul

Singlevirion Sequences

Nucleotide Diversity ∏


1.1

1

15th Nov 94

378,500

1717^{†}, 1635*

0.0012/base

1.2

1

3rd Jul 99

52,270

3331^{†}, 2514*

0.0024/base

2.1

2

22nd May 95

239,750

391^{†}, 1330*

0.0032/base

2.2

2

29th Aug 97

44,687

3747^{†}, 2504*

0.0013/base

7.1

7

19th May 95

69,180

2647^{†}

0.0022/base

7.2

7

11th Apr 00

48,083

789^{†}

0.0016/base

11.1

11

19th Jun 95

208,275

2754^{†}

0.0211/base

11.2

11

3rd Nov 98

466,200

248^{†}

0.0052/base


^{†}total number of singlevirion sequences obtained from a BAsESeq library. *total number of singlevirion sequences obtained from a PacBio library. Nucleotide diversity Π, the arithmetic mean between all pairwise differences between viral sequences within each viral population, were calculated from BAsESeq singlevirion sequences for the entire amplified genomic sequence of 3175 bases (3215 minus the 40 bases that were not amplified)