Fig. 4

TPP riboswitch influence on the reporter gfpUV gene expression of P. riograndensis SBR5. a. Schematic representation of the TPP riboswitch and TPP aptamer sequence predicted using RNAfold tool [31]; regions of riboswitch scheme in red represents possible anti-sequestering stems present in the riboswitch sequence; regions of aptamer sequence in bold are identical to the TPP riboswitch consensus sequence of B. subtilis b. GfpUV median fluorescence intensity (MFI) in SBR5 under 6 gradually increasing concentrations of thiamine; gfpUV expression was driven either by the pyk promoter with 5′ UTR exchanged by the thiC gene 5′ UTR or pyk promoter carrying native 5′ UTR. Means and standard deviation of biological triplicates were measured by flow cytometry of 20,000 cells. Under one-way between subjects ANOVA followed by post hoc comparisons using the Tukey’s HSD test, the level of significance of the differences observed in each strain between the control (0 μM of thiamine) and test conditions (5, 10, 15, 20 and 25 μM of thiamine) is represented as one star (*p ≤ 0.05). Nonsignificant differences, when p > 0.05, are not pointed