Table 3 Primers used for amplification and sequencing of the mitochondrial genome of C. cotti
Gene span | Primer name | Sequence (5′-3′) |
|---|---|---|
nad6-12S | DYF1 | TTGCGGTGCTTTGCGTTCTG |
DYR1 | CTCTCGTTTAACAGTCAACC | |
12S | XW12SF | GTTCCAGATTAATCGGCTA |
XW12SR | CAATTGATGGATGATTTGTACC | |
12S–cytb | DYF2 | CACGAGTTTAGGTTGAGCCAC |
DYR2 | CAACGTAGAGCAAGAAACC | |
cytb | XWCYTBF | GRGCTCARATGAADTATTGAGC |
XWCYTBR | TATCACTCTGGCACHAYATG | |
cytb-nad4 | DYF3 | CCTTTTCTGATGGGGGATCC |
DYR3 | GAACACAACACAGCACCCAA | |
nad4 | XWND4F | GCYCATGTTGARGCACCTAC |
XWND4R | GAAGAATARGCAGCTAAWG | |
nad4-nad1 | DYF4 | GTTATTGTCGTTTTTGGGTGC |
DYR4 | GGGTAATACAAATCACAACATCAG | |
nad1 | XWND1F | GCGTRTTGGTCCTAAYAAGG |
XWND1R | TATCAWAACGGWAACGTGGG | |
nad1-cox1 | DYF5 | GTGTTCCTTTTGTTCTACTC |
DYR5 | GAACTTCCTGACAAACAACTAC | |
cox1 | XWCOX1F | ATYGGTGGATTYGGWAATTG |
XWCOX1R | TAAACCTCNGGGTGHCCAA | |
cox1-cox2 | DYF6 | ACTATGTTGTTACTAGATCG |
DYR6 | CCACAAGGCACCACACAACG | |
cox2 | XWCOX2F | CTGAACTTATGATTACAGA |
XWCOX2R | CCACAAATCTCWGAACAYTGACC | |
cox2-16S | DYF7 | GGCAGATGCTTTGTCTGGGG |
DYR7 | TTCCGAAGACTTATCTTTG | |
16S | XW16SF | ATGGCTGYTWTAGCGTGAGG |
XW16SR | TCTATCTCACGAYGAAYTAAAC | |
16S–nad5 | DYF8 | GTAGTTCTTATACATTTTCAGG |
DYR8 | ATGGTCAACAGACCAACA | |
nad5 | XWND5F | TAGCYTTAGGYAATCACCTAC |
XWND5R | GAGACAWGGTNCTCAAWGCCAC | |
nad5 | DYF9 | GGCTTTTTGTTTTTCTGATG |
DYR9 | CTACATGCAAAATAGGTATC | |
nad5-cytb-2 | DYF10 | GGTGCTGGATAGTTATTCTGG |
DYR10 | CAATCAACTCAAAACAACAG | |
cytb-2-nad6 | DYF11 | GTTCTGTTCCTAGGAAGATC |
DYR11 | CCACAAAAACTCAACAAAGGAC | |
nad6 | DYF12 | CTATGTTTCGCTTACAACG |
DYF13 | CTACTCATAACACAAAAAC |