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Table 1 Formulation and proximate composition of the two diets

From: Transcriptomics, metabolomics and histology indicate that high-carbohydrate diet negatively affects the liver health of blunt snout bream (Megalobrama amblycephala)

 

Control

HCBD

Ingredient

 Soybean meal

35.50

34.00

 Rapeseed meal

19.00

19.00

 Wheat bran

1.20

1.20

 Fishmeal

8.00

8.00

 Wheat flour

18.00

26.30

 Soybean oil

3.80

5.20

 Lysine

0.30

0.30

 Ca(H2PO4)

2.00

2.00

 Premixa

1.00

1.00

 Choline chloride

1.00

1.00

 Carboxymethyl cellulose

2.00

2.00

 Zeolite

8.20

0.00

Proximate composition (%)

 Protein

30.84

30.28

 Lipid

7.99

8.26

 Carbohydrate

26.63

34.13

 Ashb

16.61

8.78

 Moisture

13.42

12.61

  1. aMultivitamin and mineral premix for the herbivorous fish (Wuhan Haid Group Co., Ltd): 0.5% multivitamins and 0.5% minerals. bBecause we used a high level of zeolite minerals (8.20%) in the control diet, the total ash is also much higher