Fig. 6

Diagram showing the study design. One-year-old, potted apple trees cultivars Idared and Free Redstar grafted on M.26 were inoculated with E. amylovora strain 650 in greenhouse conditions. Inoculation was made on actively growing shoots, with bacterial suspension grown overnight in TY medium. After 24 h and 6 days from the inoculation time total RNA was isolated from inoculated shoots. At each time point, RNA was isolated separately from at least 6 shoots of each apple cultivar. Additionally, RNA was isolated from the pure culture of E. amylovora 650 grown overnight in TY medium. In each time point, RNA was isolated from 3 biological replicates (in case of pure bacterial culture - two biological replicates). The replicates constituted the sample (marked by red circle)