Fig. 2From: Development of amplicon deep sequencing markers and data analysis pipeline for genotyping multi-clonal malaria infectionsSimulation of minority clone detectability by bootstrapping for marker cpmp and csp. Cut-off for acceptance of a haplotype was a minimum coverage per haplotype of 3 reads and a minority clone detection limit of 1:1000. Samples were drawn from reads of defined mixtures of P. falciparum strain 3D7 and HB3. X-axis shows dilution ratios of strains 3D7 and HB3; Y-axis indicates the sampling size (number of draws from sequence reads) for each mixture of strains. Sampling was repeated 1000 times to estimate mean minority clone detectabilityBack to article page