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Table 3 Validation of microarray analysis by RT-qPCR

From: Early nutritional programming affects liver transcriptome in diploid and triploid Atlantic salmon, Salmo salar

Genes FC Diploids V/M FC Triploids V/M
Microarray RT-qPCR p-value Microarray RT-qPCR p-value
Calpain2 (cpn2) +2.76 +1.78 >0.05 +1.44 −1.34 >0.05
Glutathione S-transferase alpha 3 (gsta3) +1.27 +1.19 >0.05 +1.99 +1.74* 0.012
Heat shock protein 4 (hspa4) −1.68 −1.45 >0.05 −1.47 −1.35 >0.05
Heat shock protein 5 (hspa5) −1.68 −1.44 >0.05 −2.49 −2.69 >0.05
Trypsin (tryp) +4.32 +3.46 >0.05 +2.39 +1.80 >0.05
Fatty acyl elongase 5 isoform b (elovl5b) +1.84 +1.53 >0.05 +1.71 +1.23 >0.05
Fatty acyl elongase 6 (elovl6) +1.96 +1.29 >0.05 +2.70 +1.73 >0.05
Delta-6 fatty acyl desaturase isoform a (fads2d6a) +1.15 +1.98 >0.05 +1.29 −1.22 >0.05
  1. Data are presented as the fold change (FC) between expression levels in diploid and triploid salmon fed the vegetable (V) diet versus the marine (M) diet. Asterisks indicate fold changes that are statistically significant (Mann-Whitney test, p < 0.05)