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Table 3 Validation of microarray analysis by RT-qPCR

From: Early nutritional programming affects liver transcriptome in diploid and triploid Atlantic salmon, Salmo salar

Genes

FC Diploids V/M

FC Triploids V/M

Microarray

RT-qPCR

p-value

Microarray

RT-qPCR

p-value

Calpain2 (cpn2)

+2.76

+1.78

>0.05

+1.44

−1.34

>0.05

Glutathione S-transferase alpha 3 (gsta3)

+1.27

+1.19

>0.05

+1.99

+1.74*

0.012

Heat shock protein 4 (hspa4)

−1.68

−1.45

>0.05

−1.47

−1.35

>0.05

Heat shock protein 5 (hspa5)

−1.68

−1.44

>0.05

−2.49

−2.69

>0.05

Trypsin (tryp)

+4.32

+3.46

>0.05

+2.39

+1.80

>0.05

Fatty acyl elongase 5 isoform b (elovl5b)

+1.84

+1.53

>0.05

+1.71

+1.23

>0.05

Fatty acyl elongase 6 (elovl6)

+1.96

+1.29

>0.05

+2.70

+1.73

>0.05

Delta-6 fatty acyl desaturase isoform a (fads2d6a)

+1.15

+1.98

>0.05

+1.29

−1.22

>0.05

  1. Data are presented as the fold change (FC) between expression levels in diploid and triploid salmon fed the vegetable (V) diet versus the marine (M) diet. Asterisks indicate fold changes that are statistically significant (Mann-Whitney test, p < 0.05)