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Table 2 Comparisons of Schizosaccharomyces pombe transcriptome assemblies of Oases, Trinity and kCombine with k=25 over different values of k-mer coverage cutoff c, with transcripts denoting the number of predicted transcripts, n50 denoting the N50 value of the length of predicted transcripts, blast denoting the number of hits from nucleotide BLAST search of predicted transcripts to different transcripts of the known transcriptome with e-value below 10−100, full denoting the number of predicted transcripts that are full length transcripts in which an entire coding region is included in a BLAST alignment, spec denoting the percentage of positions in the predicted transcripts that are included in a BLAST alignment, unique denoting the number of predicted transcripts that are uniquely mapped as reported by GMAP with the percentage in parentheses, transloc denoting the number of predicted transcripts that are translocated as reported by GMAP with the percentage in parentheses, and memory denoting the physical memory requirement as a power of 2 with Trinity using 32 CPU

From: A divide-and-conquer algorithm for large-scale de novo transcriptome assembly through combining small assemblies from existing algorithms

c

Transcripts

n50

Blast

Full

Spec

Unique

Transloc

Memory

Oases

        

10

8244

6520

6515

5623

91.9%

7268 (88.2%)

1084 (13.1%)

128 GB

20

6509

5412

6458

4597

93.1%

5959 (91.6%)

672 (10.3%)

128 GB

kCombine(Oases)

        

5

8585

2705

6227

5223

94.8%

8019 (93.4%)

646 (7.5%)

8 GB

10

6603

2620

5767

4443

95.2%

6173 (93.5%)

472 (7.1%)

8 GB

Trinity

        

5

8568

3368

6490

5279

93.7%

8228 (96.0%)

150 (1.8%)

512 GB

10

7313

2604

6415

4271

95.1%

7064 (96.6%)

156 (2.1%)

512 GB

kCombine(Trinity)

        

3

6862

2319

6152

3858

96.5%

6628 (96.6%)

262 (3.8%)

8 GB

5

6618

1938

5898

3182

97.1%

6415 (96.9%)

166 (2.5%)

8 GB