Fig. 5

Phylogenetic tree of the cowpea WRKY genes. The tree was calculated based on WRKY domain sequences of cowpea, as well as selected representative Arabidopsis and rice WRKY domains; the selection of Arabidopsis and rice WRKY sequences was based on the methodology of Li et al. (2012) [73]. This tree was generated using RAxML [62] with 100 bootstrap values with the optimal amino acid substitution model automatically chosen in RAxML (i.e., the PROTGAMMAAUTO option). The circles on the branches are bootstrap support values from 50 to 100, with the largest circles representing the greatest bootstrap support