Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness

BMC Genomics

Table 3 Sequence of the forward (F) and reverse (R) primers used in the qRT-PCR assays

Gene	Locus	Sequence (5′ – 3′)	Tm (° C)^a	Amplicon size (bp)
BoLA-DQB F	23:25,855,145–25,863,052	CATCACAGGAGCCAGAAG	80	64
BoLA-DQB R	23:25,855,145–25,863,052	GCAAACACCAATCCCAAAAT	80	64
PCP4L1 F	3:8,249,980–8,276,724	ATCTCCAGCAACCAACCAGG	82	119
PCP4L1 R	3:8,249,980–8,276,724	TCTCTGTTTCTGGTGCCGTC	82	119
GUSB F	25:28,101,456–28,233,126	CAGGGCGGGATGCTCTA	84	93
GUSB R	25:28,101,456–28,233,126	GTTGTCGGAGAAGTCGGC	84	93
HPRT1 F	3:103,318,856–103,327,860	TGATGAAGGAGATGGGTGGC	83	81
HPRT1 R	3:103,318,856–103,327,860	CCAACAGGTCGGCAAAGAAC	83	81
TBP F	9:105,686,118–105,690,814	ATAGTGTGCTGGGGATGCTC	80	114
TBP R	9:105,686,118–105,690,814	GTGGGAGGCTGTTGTTCTGA	80	114

ISSN: 1471-2164