Fig. 4

Comparison of the median expression levels of transcriptional target genes. The median expression levels of the target genes of the same transcription factor binding sequences were compared between promoters and three types of promoter and extended regions for enhancer-promoter association (EPA). Red and blue dots show statistically significant difference of the distribution of expression levels of target genes between promoters and EPA. Red dots show the median expression level of target genes was higher in EPA than promoters, and blue dots show the median expression level of target genes was lower in EPA than promoters. The median expression levels of putative transcriptional target genes were significantly lower using TF bound in enhancers than using TF bound in promoters in stem cells such as H1-hESC and iPSC, and higher by TF bound in enhancers in immune cells. These results implied that enhancers significantly affect the expression of target genes