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Table 3 Microarray validation by qPCR for the HT-E2 vs. HT comparison

From: Estrogen exposure overrides the masculinizing effect of elevated temperature by a downregulation of the key genes implicated in sexual differentiation in a fish with mixed genetic and environmental sex determination

Microarray qPCR
Gene symbol log2 Fold change Adjusted P -value log2 Fold change Adjusted P-value
amh −2.48 0.037* −3.18 0.011*
aqp1 2.16 0.349 0.70 0.094
col18a −4.28 0.035* −0.78 0.174
cyp19a1a −1.03 0.781 −3.47 0.000***
cyp19a1b −1.37 0.089 0.12 0.028*
dmrt1 −1.54 0.007** −2.39 0.004**
gnrh 1.11 0.129 −1.83 0.051
igf1 5.95 0.035* −1.73 0.149
mettl22 −1.31 0.121 −1.21 0.113
prl −1.03 0.806 −1.94 0.018*
sox17 1.08 0.779 0.35 0.570
star −1.87 0.051 −2.00 0.006**
tesc −1.17 0.324 −1.88 0.017*
vasa −0.64 0.721
wisp1 1.02 0.816 −1.51 0.045*
  1. Note: Asterisks note statistical significant differences: * = P < 0.05; ** = P < 0.01; *** = P < 0.001. N 10 individuals analyzed per gene