A next generation sequencing based approach to identify extracellular vesicle mediated mRNA transfers between cells

Table 4 Estimate mRNA transfer rates in in vitro co-culture

Direction	Rates	Simulation			Real Co-culture			KS distance^c
Direction	Rates	# Loci^a	#Sig Loci^b	Sig Ratio	#Loci	#Sig Loci	Sig Ratio	KS distance^c
MO to AD	0.1	83,984	41,999	5.00E-1	80,969	972	1.20E-2	0.535
	0.01	73,838	6288	8.52E-2	74,056	841	1.14E-2	0.140
	0.001	73,037	513	7.02E-3	73,441	831	1.13E-2	0.047
	0.0001	72,958	107	1.47E-3	73,364	853	1.16E-2	0.066
AD to MO	0.1	225,698	61,305	2.72E-1	235,323	1293	5.49E-3	0.320
	0.01	215,598	7965	3.69E-2	223,395	1216	5.44E-3	0.058
	0.001	214,592	635	2.96E-3	216,059	1174	5.43E-3	0.025
	0.0001	214,509	58	2.70E-4	221,935	1202	5.42E-3	0.034

^a The number of overall loci checked in 10 simulated (down-sampled real) samples
^b The number of overall significant loci (Bayesian factor > = 20) in 10 simulated (down-sampled real) samples
^c The KS distance between the Bayesian factor distribution for simulated data and that for real co-culture data as calculated by ks.test in R

ISSN: 1471-2164