Fig. 1

Construction of RCc3:nMYC:OsNAC or RCc3:OsNAC:cMYC transgenic rice plants. a Map of the binary vector used for generating RCc3:OsNAC5, 6, 9, and 10 transgenic rice lines. RB, right border; pRCc3, a root specific promoter [48]; tNOS, NOS terminator; Bar, phosphinothricin acetyltransferase gene, an herbicide resistance gene used as a selection marker; LB, left border. Orange color indicates nMYC (N-terminal portion of 6MYC protein) or cMYC (C-terminal portion of 6MYC protein). b Translational expression of OsNAC5, 6, 9, and 10 proteins fused cMYC or nMYC, as measured by Western blot analysis using an anti-MYC antibody. Protein extracts from roots of twenty-day-old nontransgenic wild type (NT, Oryza sativa L. japonica cv. Illmi) and RCc3: OsNAC5, 6, 9, and 10 transgenic seedlings (10 μg per lane) were subjected to SDS-PAGE (polyacrylamide gel electrophoresis) and Western blot analysis. Coomassie blue staining is shown to confirm equal loading. Red figure, definite overexpressed 6MYC fused OsNAC protein