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Table 1 List of bioinformatics tools and resources currently incorporated within A-GAME

From: A-GAME: improving the assembly of pooled functional metagenomics sequence data

Quality Trimming

 Tool

Reference

  FastQC

[30]

  Pear

[31]

  Flash

[32]

  Trimmomatic

[33]

  FastX

[34]

 Assembly

  Megahit

[35]

  SPAdes

[36]

  Abyss

[37]

  Velvet

[38]

  Meta-Velvet

[39]

  Meta-SPAdes

[40]

 Gene prediction

  Glimmer

[41]

  Augustus

[42]

  Prokka (Prodigal)

[43]

  Metagenemark

[28]

 Functional annotation

  Interpro

[44]

  PFAM

[45]

  Blast + suite

[46]

 Short read mapping

  Bowtie2

[47]

  bwa

[48]

 Scaffolding

  SSpace

[23]

  Sopra

[49]