Fig. 3
DNA damage and DDR activation upon CDC6 induction are reduced in the “escaped” cells. a Cell cycle analysis upon CDC6 induction and plot of re-replicating cells (DNA content > 4n). b Comet assay showed DNA breaks in cells induced for the indicated time points. DNA damage was significantly reduced in the “escaped” cells. Plot depicts tail comet (moment) calculations. Red lines depict moment tails. Scale bar: 35 μm. c DDR activation in CDC6-induced cells as demonstrated by double IF for 53BP1 and CDC6 (Scale bar: 20 μm) along with western blotting for p53 and p21WAF1/Cip1. DDR is reduced in the “escaped” cells. Actin serves as a loading control.