Fig. 1
GISH analysis of chromosome components in the progenies of the Gossypium hirsutum-G. australe pentaploid (2n = AADDG2) pollinated by irradiation-induced pollen. a. Mitotic chromosome spread of the 52 chromosomes of G. hirsutum. b. Mitotic chromosome spread of the 26 chromosomes of G. australe. c- f. Mitotic chromosome spread showing the 52 G. hirsutum chromosomes (blue signals) plus one (1G), two (5G, 8G), three (3G, 9G, 12G) and four (6G, 7G, 8G, 9G) chromosomes of G. australe (red signals, white arrowhead). g-i. GISH revealing the G. hirsutum-G. australe translocation chromosomes (white arrowhead). The gDNA of G. australe was labeled with digoxigenin-11-dUTP and chromosomes from G. australe were visualized as red signals; the gDNA of G. herbaceum was labeled with biotin-16-dUTP and the resultant A-subgenome chromosomes in G. hirsutum were visualized as green signals; D-subgenome chromosomes in G. hirsutum were counterstained with 4, 6-diamidino-2-phenylindole (DAPI) and were visualized as blue signals