Fig. 6
From: Soybean MADS-box gene GmAGL1 promotes flowering via the photoperiod pathway
Expression of soybean maturity genes in transgenic and WT plants during three periods. The expression of genes in SAM in the VC, V4 and R1 periods in GmAGL1-OX line 1, line 5 and WT were assessed. Gene transcript levels were determined by qRT-PCR. The α-Tubulin gene was amplified as an internal control to normalize the data. Transcript levels were calculated using the 2-ΔΔCt formula to determine their expression levels relative to the standard. Black squares (with a black solid line) indicate gene transcript expression in AGL1-OX line 1. Light grey triangles (with a light grey dotted line) indicate gene transcript expression in AGL1-OX line 5. Dark grey circles (with a dark grey dotted line) indicate gene transcript expression in WT. Data are presented as the means of three biological replicates, and error bars indicate SDs. Asterisks indicate a significant difference between WT and transgenic plants (**P < 0.01, *P < 0.05)