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Fig. 1 | BMC Genomics

Fig. 1

From: Unique, dual-indexed sequencing adapters with UMIs effectively eliminate index cross-talk and significantly improve sensitivity of massively parallel sequencing

Fig. 1

Unique dual-matched UMI adapters are compatible with shear ligation library construction methods and may be sequenced in three different modes depending on the sensitivity of the application. a For genotyping applications, the i7 sample index may be used for demultiplexing. b More sensitive applications, such as somatic variant calling, should use the i7 and i5 index. c When a unique molecular identifier (UMI) is required for greater sensitivity, the read length for the i7 index may be increased to sequence the sample index and UMI in addition to the i5 sample index

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BMC Genomics

ISSN: 1471-2164

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