Table 2 Primer characteristics
From: In-depth proteomic analysis of boar spermatozoa through shotgun and gel-based methods
Gene names | NCBI Accession # | Primer sequences (5’➔3′) | Product size(bp) |
|---|---|---|---|
Water aquaporins | |||
AQP-1 | NM_214454 | FP: CCGGCAACTCCCTTGGCCTG RP: GGGTTAATGCCGCAGCCGGT | 220 |
AQP-5 | NM_001110424 | FP: TGGGCTGGCACCTGGCAATG RP: CGACTGCGGGGCCGAAAGAG | 266 |
AQP-11 | NM_001112682 | FP: TGCGTGGGTGCCTTGTGGAG RP: GTGCAGCAAAGCGCTGTGGAA | 150 |
Aquaglyceroporins | |||
AQP-3 | NM_001110172 | FP: TCGTGTGCGTGCTGGCCATT RP: ACCGGCGATGGAACCCAGGA | 257 |
AQP-7 | NM_001113438 | FP: AGTGACCGGTCCCACAGCCA RP: CTGTGTGCCCCAGCCAGCAA | 295 |
AQP-9 | NM_001112684 | FP: GGGCTGCAACCCTCTTTGGCA RP: TGGGCTGTAGGCCTCTGGGGA | 234 |
AQP-10 | NM_001128454 | FP: GGGTCTGTGGCCCAGGCAGTA RP: TGGCCAGGGAGAAGGCTGGA | 148 |
Spermadhesins | |||
AQN-1 | NM_001025210 | FP: CAGGCTGCTGAGATGAAGCTGGGC RP: CGCAGGCGAGGTTGAGATACGG | 235 |
AWN | NM_213829 | FP: GTCCTCAGAGACCCTCCTGGGAA RP: GGAAGGGAGAGGCACGCTGG | 277 |
PSP-I | NM_213837 | FP: GCGGTTCTTCAGGCATGACGGT RP: GTGGCCAGGAGATGGCGCTC | 203 |
PSP-II | NM_213836 | FP: ACTGCCATCCCCTGGGCCTT RP: GCCGTAGACAAAAAGACAATCGCTG | 305 |
SPMI | NM_001031776 | FP: TCCGAGACCAACGGGCAGGAC RP: TGGCTCCTTGTGGGATGCCGT | 165 |
Glucose transporters | |||
GLUT-3 | FJ_209733 | FP: GGGGGCCTTTGGCACTCTCA RP: AAGCCCAAGAGCAGGGGCCA | 117 |
GLUT-5 | EU_012359 | FP: TCGGCTCCCTCATGGTCGGC RP: TGGGCGACTTTGCTGCATCCC | 118 |