Fig. 5
From: The invasive MED/Q Bemisia tabaci genome: a tale of gene loss and gene gain
Functional validation of detoxification enzymes using dietary RNAi. The genomic structure of CYP4s (CYP304G2, CYP402C9, CYP4CR2 and CYP4G69), CYP6s (CYP6CX4, CYP6DB3, CYP6DV6, CYP6DW2 and CYP6EM1), and GSTs (GSTD6, GSTD9 and GSTM1) are respectively shown in panels (a), (c), and (e). Images from gel electrophoresis of PCR and qRT-PCR products are displayed within the inset pictures. Plots of survival rates of CYP4, CYP6, and GST knockouts when exposed to 0.2 mM imidacloprid are documented in (b), (d), and (f), respectively (* p < 0.05; ** p < 0.01). Control treatments include non-insecticide (−, buffer, EGFP and genes), vehicle (+, buffer), and control gene (+, EGFP). Buffer are an aqueous artificial diet solution containing 5% yeast extract and 30% sucrose (wt/vol)