Fig. 7

Transcription profile of the phage island and the tox gene. a The transcription profile of the phage island (framed by two red lines) of the wild type and the isogenic ΔdtxR mutant is shown. The genes which could be assigned with a TSS are labeled with a black arrow. Primary operon structures are indicated by dashed arrows. The tox gene is shown in dark yellow. TSS assigned to intergenic and antisense transcripts were omitted for clarity. b Detailed view on the monocistronic tox gene. It possesses two TSS (TSS 1 and TSS 2) with 5’-UTR lengths of 43 nt and 38 nt. The DtxR binding site (red box) is located close to the start codon of the CDS and overlaps both TSS and their corresponding −10 promoter motifs. Two additional TSS for putative antisense RNAs were found (light green arrows) and two additional TSS (intergenic TSS 1 and intergenic TSS 2) on the forward and reverse strand, respectively, were identified upstream of the tox gene, indicating two putative novel transcripts. The image is a modified screenshot from ReadXplorer [48] showing non-normalized coverage data of one exemplary replicate per condition