Fig. 10

FuSpot Visualization of DNAJC21-CNGA1 False Positive Fusion Junction Reported by EricScript. FuSpot’s visualization of the putative DNAJC21-CNGA1 fusion gene claimed by EricScript when given the BEERS data set [25]. Each read seen here was extracted from either the normal or chimeric output file produced by aligning the BEERS [25] data set with STAR. All reads were extracted using the FuSpot extraction tool available on the FuSpot website. The reads in Group 1 all align normally across the DNAJC21 partners and the majority of reads in Group 2 align in the congruent manner across the CNGA1 partners. However, through FuSpot’s realignment, the first read of Group 2 reveals that there is sequence homology along the last few base pairs of the 5′ references; this is likely what caused EricScript to falsely report the fusion point