Fig. 5

FuSpot Visualization of ACACA-STAC2 True Fusion Gene Using FusionCatcher Supporting Reads. An alternative visualization produced by FuSpot of the lab-validated ACACA-STAC2 fusion gene from Edgren et al. [22] Each read seen here was reported by the FusionCatcher fusion detector tool when run on the data set from Edgren et al. [22] Unlike FusionMap, FusionCatcher provides in its output supporting reads and available mates which span or flank the breakpoint. Here, several reads span the breakpoint, some with a spanning first mate and some with a spanning second. Anchor lengths over the breakpoint vary from a few base pairs to about half the length of the read. Two reads flank the breakpoint. This distribution provides clear and present evidence that the ACACA-STAC2 fusion is a true positive