Summarizing performance for genome scale measurement of miRNA: reference samples and metrics

BMC Genomics

Table 2 Summary metrics derived from using pure samples from Round 3 for baseline predictions

Measurement		Data Source			miRNA Detected				Log2 Signals			AUC^c		Deviation
Process	Technology	by Round			Non-	TrueNeg	TruePos		Lower	Upper	Range	All	Within	Log2 Ratio		Proportion
		Pure	Mixes	Reps	Selective	1-to-1^a	Brain	Placenta	Limit^b	Limit	Range	All	Range	Median	IQR	Sum
A	NGS	3	3	3	928	56	103	104	5.8	20.0	14.3	0.959	0.996	-0.013	0.303	0.375
			4	3					5.4	20.2	14.8	0.966	0.988	-0.006	0.311	0.358
			5	3					6.3	20.0	13.8	0.899	0.938	-0.059	0.380	0.361
B	NGS	3	3	3	868	56	134	47	7.3	20.2	12.8	0.905	0.953	-0.085	0.387	0.508
			4	3					6.3	19.5	13.2	0.866	0.932	-0.092	0.399	0.448
			5	3					6.7	19.7	12.9	0.848	0.904	-0.435	0.397	0.565
C	NGS	3	3	3	421	35	29	49	5.3	16.3	11.0	0.944	0.971	0.030	0.266	0.332
			4	3					11.1	16.1	5.0	0.831	0.850	0.040	0.626	0.460
			5	3					15.7	15.7	0.0	0.848	ND^d	-0.124	0.839	0.556
D	NGS	3	3	3	248	16	18	9	8.6	10.6	2.0	0.781	ND^d	-0.124	0.627	0.803
D	NGS	3	4	3	248	16	18	9	6.1	10.7	4.6	0.956	1.000	-0.178	0.539	0.645
E	Digital Hybridization	3	3	3	693	65	8	24	7.1	13.3	6.1	0.947	1.000	-0.075	0.392	0.460
			4	3					7.7	13.7	6.0	0.949	1.000	-0.260	0.495	0.532
			5	3					13.2	14.4	1.1	0.925	ND^d	-0.257	0.680	0.579

^aLiver-selective miRNA or miRNA with approximately equal signals in both brain and placenta
^bLower limits for NGS and digital hybridization were calculated with at 95% of values falling within ± 0.585 log2 difference from mean
^cROC curves were prepared with P-values using three replicates of Mix1 and Mix2
^dNot determined (ND) due to insufficient number of true negative or true positive values

ISSN: 1471-2164