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Table 3 Summary metrics derived from using one replicate of each sample per round

From: Summarizing performance for genome scale measurement of miRNA: reference samples and metrics

Measurement   Data Source miRNA Detected Log2 Signals AUCc Deviation
Process Technology by Round   Non- TrueNeg TruePos Lower Upper Range All Within Log2 Ratio Proportion
   Pure Mixes Reps Selective 1-to-1a Brain Placenta Limitb Limit Range Median IQR Sum
A NGS 3 3 1 671 43 93 89 7.1 20.0 12.9 0.856 1.000 0.094 0.386 0.394
4 4 1 696 51 98 91 8.0 20.2 12.2 0.921 0.996 0.081 0.492 0.399
5 5 1 692 36 96 95 9.5 20.2 10.8 0.793 0.971 -0.102 0.545 0.323
B NGS 3 3 1 618 35 109 37 8.9 20.1 11.2 0.832 0.969 -0.055 0.405 0.559
4 4 1 527 35 97 33 8.0 19.0 11.0 0.910 0.972 -0.106 0.384 0.417
5 5 1 455 28 73 32 7.8 19.2 11.4 0.849 1.000 -0.031 0.354 0.456
C NGS 3 3 1 326 11 26 39 6.6 16.3 9.7 0.905 0.983 0.030 0.323 0.348
4 4 1 158 12 15 4 8.4 11.8 3.5 0.704 1.000 0.001 0.617 0.382
5 5 1 247 13 18 15 12.0 12.5 0.5 0.795 NDd -0.031 0.756 0.498
D NGS 3 3 1 151 6 18 3 10.5 0.648 NDd -0.235 0.969 0.644
4 4 1 205 13 20 3 7.6 10.5 3.0 0.903 0.950 -0.126 0.569 0.471
E Digital Hybridization 3 3 1 702 63 6 19 13.2 13.4 0.2 0.898 NDd -0.077 0.661 0.635
4 4 1 618 27 12 24 14.6 15.4 0.8 0.727 NDd -0.340 0.670 0.376
5 5 1 599 38 17 37 7.5 15.3 7.9 0.774 1.000 -0.165 0.749 0.511
  1. aLiver-selective miRNA or miRNA with approximately equal signals in both brain and placenta
  2. bLower limits for NGS and digital hybridization were calculated with at 95% of values falling within ± 0.585 log2 difference from mean
  3. cROC curves were prepared using ordered log2 ratios and a single replicate
  4. dNot determined (ND) due to insufficient number of true negative or true positive values