Fig. 2

Features of spermatogenic promoters in O. dioica. a Heatmap shows the density of TCTAGA at each position (x-axis) in a -500 to +500 bp region centred on the male dominant TSS for spermatogenic promoter sequences (rows) ordered by promoter width (top to bottom = broad to sharp). Darker blue indicates higher enrichment. b Distribution of distances (median = 44 bp) to the TCTAGA motif relative to the dominant TSS in male day 6 animals. c The TCTAGA motif is specific to transcription in the adult male (final bar in each plot = male-specific (4_4) cluster): of all promoters with a TCTAGA motif the majority have a male-specific expression profile (top). Moreover, the majority of spermatogenic transcription is associated with a TCTAGA motif: of all promoters with a male-specific expression profile the majority contain a TCTAGA, whereas very few promoters in other expression classes contain this motif (bottom). d Distribution of tag counts (tpm) for different promoter classes (see colour key code in e) in the male: TATA = TSS with upstream TATAW element; TCTAGA = TSS with upstream TCTAGA; Sharp = all other promoters with narrow region of TSSs; Broad = dispersed region of TSSs. e ChIP-chip data for day 6 testes are shown for RNAPII, H3K18ac, H3K27ac, H3K4me3, H3K4me2, H3K36me1 and H3K9me1. Each plot shows the mean log₂ ratio of ChIP/input at each probe position in a 1000 bp window centered on the dominant TSS. Promoters were categorized according to promoter type as in d. Error bars show 95% confidence intervals for the mean obtained by bootstrapping