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Fig. 1 | BMC Genomics

Fig. 1

From: SALP, a new single-stranded DNA library preparation method especially useful for the high-throughput characterization of chromatin openness states

Fig. 1

Schematic of tagmentation-based SALP-seq. a Barcoded Tn5 adaptor (BTA) used in SALP-seq. BTA consists of a 19-bp double-stranded transposase binding site (ME) and a single-stranded barcode and PCR primer annealing site. The Tn5·BTA complex (transposome) is used to tagment DNA or chromatin. b SALP-seq experimental procedures. Single strand adaptor (SSA) is a double-stranded oligonucleotide with a 3′ overhang, which is 3 random nucleotides (3N). c Tagmentation-based SALP-seq strategy for high-throughput characterization of chromatin openness states of multiple cells

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BMC Genomics

ISSN: 1471-2164

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