Table 2 Oligos used in this study
From: UDiTaS™, a genome editing detection method for indels and genome rearrangements
Oligo Name | Description | Sequence |
|---|---|---|
OLI7076 | Forward primer for B2M IVT | CACCGCTAGCTAATACGACTCACTATAGGCCACGGAGCGAGACATCTGTTTTAGAGCTAGAAATA |
OLI7077 | Forward primer for TRAC5 IVT | CACCGCTAGCTAATACGACTCACTATAGCTGGTACACGGCAGGGTCAGTTTTAGAGCTAGAAATA |
OLI4610 | Forward primer for TRAC5 Illuimina amplicon sequencing | ACACTCTTTCCCTACACGACGCTCTTCCGATCTGCATTTCAGGTTTCCTTGAGTGG |
OLI4611 | Reverse primer for TRAC5 Illuimina amplicon sequencing | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGCACTGTTGCTCTTGAAGTCC |
OLI7078 | Common reverse primer for IVT template | TTTTTTTTTTTTTTTTTTTTGCACCGACTCGGTGCCACTTTTTCAAGTTGATA |
OLI6062 | UDiTaS and AMP-seq gene specific primer for CEP290 guide 323 | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGGACCATGGATGCACTCTGTAAATTCTCAT |
OLI6256 | UDiTaS and AMP-seq gene specific primer for B2M | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGCATGCCTTCTTAAACATCACGAGACTCTAA |
OLI6253 | UDiTaS and AMP-seq gene specific primer for TRAC5 - Forward | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGGTTTCTAAGATGCTATTTCCCGTATAAAGCATGA |
OLI6259 | UDiTaS and AMP-seq gene specific primer for TRAC5 - Reverse | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGCACTGTTGCTCTTGAAGTCCATAGACCTC |
OLI6380 | UDiTaS adapter top oligo i5_N501_UMI_Tn5-A | AATGATACGGCGACCACCGAGATCTACACTAGATCGCNNNNNNNNNNTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG |
OLI6381 | UDiTaS adapter top oligo i5_N502_UMI_Tn5-A | AATGATACGGCGACCACCGAGATCTACACCTCTCTATNNNNNNNNNNTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG |
OLI6382 | UDiTaS adapter top oligo i5_N503_UMI_Tn5-A | AATGATACGGCGACCACCGAGATCTACACTATCCTCTNNNNNNNNNNTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG |
OLI6383 | UDiTaS adapter top oligo i5_N504_UMI_Tn5-A | AATGATACGGCGACCACCGAGATCTACACAGAGTAGANNNNNNNNNNTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG |
OLI6384 | UDiTaS adapter top oligo i5_N505_UMI_Tn5-A | AATGATACGGCGACCACCGAGATCTACACGTAAGGAGNNNNNNNNNNTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG |
OLI6385 | UDiTaS adapter top oligo i5_N506_UMI_Tn5-A | AATGATACGGCGACCACCGAGATCTACACACTGCATANNNNNNNNNNTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG |
OLI6386 | UDiTaS adapter top oligo i5_N507_UMI_Tn5-A | AATGATACGGCGACCACCGAGATCTACACAAGGAGTANNNNNNNNNNTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG |
OLI6387 | UDiTaS adapter top oligo | AATGATACGGCGACCACCGAGATCTACACCTAAGCCTNNNNNNNNNNTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG |
Tn5-A bottom | UDiTaS adapter bottom oligo | [Phos]CTGTCTCTTATACA[ddC] |
OLI5589 | UDiTaS and AMP-seq round 1 and 2 PCR primer P5/i5 | AATGATACGGCGACCACCGAGATCTACAC |
OLI5639 | UDiTaS and AMP-seq round 2 PCR primer i7_N701_SBS12 | CAAGCAGAAGACGGCATACGAGATAGCGGAATGTGACTGGAGTTCAGACGTGT |
OLI5640 | UDiTaS and AMP-seq round 2 PCR primer i7_N702_SBS12 | CAAGCAGAAGACGGCATACGAGATGATCATGCGTGACTGGAGTTCAGACGTGT |
OLI5641 | UDiTaS and AMP-seq round 2 PCR primer i7_N703_SBS12 | CAAGCAGAAGACGGCATACGAGATAAGACGGAGTGACTGGAGTTCAGACGTGT |
OLI5642 | UDiTaS and AMP-seq round 2 PCR primer i7_N704_SBS12 | CAAGCAGAAGACGGCATACGAGATCGAGTCCTGTGACTGGAGTTCAGACGTGT |
OLI5643 | UDiTaS and AMP-seq round 2 PCR primer i7_N705_SBS12 | CAAGCAGAAGACGGCATACGAGATTCCTCAGGGTGACTGGAGTTCAGACGTGT |
OLI5644 | UDiTaS and AMP-seq round 2 PCR primer i7_N706_SBS12 | CAAGCAGAAGACGGCATACGAGATGTACGGATGTGACTGGAGTTCAGACGTGT |
OLI5645 | UDiTaS and AMP-seq round 2 PCR primer i7_N707_SBS12 | CAAGCAGAAGACGGCATACGAGATCATCTCTCGTGACTGGAGTTCAGACGTGT |
OLI5646 | UDiTaS and AMP-seq round 2 PCR primer i7_N710_SBS12 | CAAGCAGAAGACGGCATACGAGATGTCGGAGCGTGACTGGAGTTCAGACGTGT |
OLI5647 | UDiTaS and AMP-seq round 2 PCR primer i7_N711_SBS12 | CAAGCAGAAGACGGCATACGAGATACGGAGAAGTGACTGGAGTTCAGACGTGT |
OLI5648 | UDiTaS and AMP-seq round 2 PCR primer i7_N712_SBS12 | CAAGCAGAAGACGGCATACGAGATAGGAGATGGTGACTGGAGTTCAGACGTGT |
OLI5649 | UDiTaS and AMP-seq round 2 PCR primer i7_N714_SBS12 | CAAGCAGAAGACGGCATACGAGATAGTACTCGGTGACTGGAGTTCAGACGTGT |
OLI5650 | UDiTaS and AMP-seq round 2 PCR primer i7_N715_SBS12 | CAAGCAGAAGACGGCATACGAGATGGACTCTAGTGACTGGAGTTCAGACGTGT |
OLI2909 | Index 1 - AMP-seq top adapter | AATGATACGGCGACCACCGAGATCTACACACTGCATANNWNNWNNACACTCTTTCCCTACACGACGCTCTTCCGATC*T |
OLI2910 | Index 1 - AMP-seq top adapter | AATGATACGGCGACCACCGAGATCTACACAAGGAGTANNWNNWNNACACTCTTTCCCTACACGACGCTCTTCCGATC*T |
Illumina forward | Round 2 barcode primer for Illumina amplicon sequencing | AATGATACGGCGACCACCGAGATCTACACNNNNNNNNACACTCTTTCCCTACACGAC |
Illumina reverse | Round 2 barcode primer for Illumina amplicon sequencing | CAAGCAGAAGACGGCATACGAGATNNNNNNNNGTGACTGGAGTTCAGACGTGT |
AMP-seq bottom adapter | AMP-seq bottom adapter | [Phos]GATCGGAAGAGC*C*A |