Fig. 4From: Transcriptome-wide identification of optimal reference genes for expression analysis of Pyropia yezoensis responses to abiotic stressComparison of Normalized expression level of PyOLE-1 gene between single and the best reference genes under temperature stress. (a) Relative quantification of PyOLE-1 gene expression using the single reference gene MAP and the best stable reference genes (MAP, UBC and CGS1) under high temperature (a), chilling temperature (b) and freezing temperature (c) respectively. The average Ct value was calculated from three biological and technical replicates and used for relative expression analysesBack to article page