Fig. 5

Identification of putative SOX10 response elements in Schwann cells. a All SOX10 consensus sequences in the human genome were identified, and these data were intersected with multiple-species conserved sequences (MCSs; see text for details). Overlap between the conserved SOX10 monomers and SNPs validated ‘by-frequency’ from dbSNP 130 was determined, and exons were excluded using RefSeq entries. This dataset of conserved, non-coding SOX10 monomers harboring a SNP was prioritized by identifying regions overlapping SOX10 ChIP-Seq data or by identifying a dimeric SOX10 consensus sequence. The number of regions in each resulting dataset is indicated under the label. b and c The 22 genomic segments from panel A were cloned upstream of a luciferase reporter gene and tested in the forward (b; blue bars) and reverse (c; red bars) orientation in SOX10-positive S16 cells. The activity of each genomic segment is expressed relative to a control vector with no genomic insert (‘Empty’ in b and c). Four regions displayed greater than five-fold activity (indicated by the dashed line) in at least one orientation. Error bars indicate standard deviations