Fig. 3

nrdAB-VC1254 expression. a. Marine RNA-seq data in presence (+) or absence of mitomycin C (−). The top tracks correspond to the piling up signal of all reads. The middle tracks correspond to individual reads. Arrow corresponds to transcriptional start. The bottom track shows the gene annotation of Vibrio cholerae. b. RT-PCR of the entire operon in presence (+) and absence (−) of AMV reverse transcriptase. MT corresponds to 1Kb ladder. c. EMSA performed using nrdAB promoter region (VC-Nrd) in presence (+) and absence (−) of LexA protein. A positive SOS-box probe was included as a control