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Table 1 Quantitative real time PCR (qPCR) validation of sequencing (Seq) results

From: Transcriptional profiling of swine mammary gland during the transition from colostrogenesis to lactogenesis using RNA sequencing

 

-14 vs −2 time comparison

-14 vs +1 time comparison

Target

FDR

FC

P-value

FC

P-value

CSN2

 qPCR

<.0001

51.9

<.0001

254.8

<.0001

 Seq

0.001

31.0

0.003

33.5

<.0001

LALBA

 qPCR

<.0001

156.1

<.0001

8321.9

<.0001

 Seq

0.003

128.5

0.03

1275.5

0.001

LTF

 qPCR

<.0001

1.5

0.02

3.4

<.0001

 Seq

0.001

1.7

0.10

3.9

<.0001

PAEP

 qPCR

0.0001

2.2

0.0005

3.4

<.0001

 Seq

0.004

1.8

0.01

1.9

0.001

  1. The overall false discovery rate (FDR) together with the fold-change (FC) and P-value for the specific comparison is reported for each gene. The P-values were generated applying the same statistical model to either qPCR or Seq data
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BMC Genomics

ISSN: 1471-2164

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