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Table 1 Quantitative real time PCR (qPCR) validation of sequencing (Seq) results

From: Transcriptional profiling of swine mammary gland during the transition from colostrogenesis to lactogenesis using RNA sequencing

  -14 vs −2 time comparison -14 vs +1 time comparison
Target FDR FC P-value FC P-value
CSN2
 qPCR <.0001 51.9 <.0001 254.8 <.0001
 Seq 0.001 31.0 0.003 33.5 <.0001
LALBA
 qPCR <.0001 156.1 <.0001 8321.9 <.0001
 Seq 0.003 128.5 0.03 1275.5 0.001
LTF
 qPCR <.0001 1.5 0.02 3.4 <.0001
 Seq 0.001 1.7 0.10 3.9 <.0001
PAEP
 qPCR 0.0001 2.2 0.0005 3.4 <.0001
 Seq 0.004 1.8 0.01 1.9 0.001
  1. The overall false discovery rate (FDR) together with the fold-change (FC) and P-value for the specific comparison is reported for each gene. The P-values were generated applying the same statistical model to either qPCR or Seq data