Table 1 Number of miRNAs differentially expressed in RNA sequencing validated by Qiagen miRnome high throughput qPCRa
From: Evaluation of commercially available small RNASeq library preparation kits using low input RNA
Kit | Tissue Comparison | Differentially Expressed miRNAs (RNASeq)a | Assayed by qPCR | Concordant – in same direction as RNASeq (%) | Discordant – in opposite direction as RNASeq (%) |
|---|---|---|---|---|---|
BiooScientific | B_vs_L | 322 | 285 | 209 (73.33%) | 76 (26.67%) |
BiooScientific | B_vs_P | 366 | 305 | 299 (98.03%) | 6 (1.97%) |
BiooScientific | P_vs_L | 317 | 269 | 214 (79.55%) | 55 (20.45%) |
Illumina-TruSeq | B_vs_L | 227 | 207 | 164 (79.23%) | 43 (20.77%) |
Illumina-TruSeq | B_vs_P | 295 | 254 | 250 (98.43%) | 4 (1.57%) |
Illumina-TruSeq | P_vs_L | 241 | 206 | 162 (78.64%) | 44 (21.36%) |
NEB | B_vs_L | 250 | 226 | 186 (82.3%) | 40 (17.7%) |
NEB | B_vs_P | 312 | 269 | 262 (97.4%) | 7 (2.6%) |
NEB | P_vs_L | 262 | 219 | 179 (81.74%) | 40 (18.26%) |