Table 3 Number of miRNAs differentially expressed in RNA sequencing validated by FirePlexa
From: Evaluation of commercially available small RNASeq library preparation kits using low input RNA
Kit | Tissue Comparison | Differentially Expressed miRNAs (RNASeq)a | Assayed by FirePlex | Concordant – in same direction as RNASeq (%) | Discordant – in opposite direction as RNASeq (%) | Concordant and Significant (%) |
|---|---|---|---|---|---|---|
BiooScientific | B_vs_L | 322 | 47 | 39 (82.98%) | 8 (17.02%) | 28 (59.57%) |
BiooScientific | B_vs_P | 366 | 54 | 52 (96.3%) | 2 (3.7%) | 35 (64.81%) |
BiooScientific | P_vs_L | 317 | 42 | 41 (97.62%) | 1 (2.38%) | 32 (76.19%) |
Illumina-TruSeq | B_vs_L | 227 | 43 | 39 (90.7%) | 4 (9.3%) | 29 (67.44%) |
Illumina-TruSeq | B_vs_P | 295 | 53 | 50 (94.34%) | 3 (5.66%) | 35 (66.04%) |
Illumina-TruSeq | P_vs_L | 241 | 38 | 38 (100%) | 0 (0%) | 31 (81.58%) |
NEB | B_vs_L | 250 | 45 | 39 (86.67%) | 6 (13.33%) | 29 (64.44%) |
NEB | B_vs_P | 312 | 51 | 50 (98.04%) | 1 (1.96%) | 34 (66.67%) |
NEB | P_vs_L | 262 | 39 | 39 (100%) | 0 (0%) | 31 (79.49%) |