Table 2 A catalogue of downregulated proteins identified using iTRAQ labeling method An. culicifacies in species B
S.no | UniProt no. | Protein | ∑ coverage | Peptides | M.wt (kDa) | Function | Ratio |
|---|---|---|---|---|---|---|---|
1. | B0WZ30 | Histone H2B (similar to Culex quinquefasciatus) | 32 | 5 | 13.8 | DNA binding | 0.61 |
2. | Q1HQX3 | AAEL012326-PA (similar to Aedes aegypti) | 31 | 6 | 16.8 | calcium ion binding | 0.62 |
3. | F5HME4 | AGAP001799-PB (similar to Anopheles gambiae) | 24 | 8 | 32.4 | Unknown | 0.61 |
4. | Q7PJV2 | AGAP010147-PA (similar to Anopheles gambiae) | 23 | 56 | 224.2 | ATP binding, motor activity | 0.60 |
5. | B0X773 | Histone H2A (similar to Culex quinquefasciatus) | 20 | 4 | 13.3 | DNA binding | 0.43 |
6. | Q5TMR7 | AGAP011939-PA (similar to Anopheles gambiae) | 20 | 7 | 35.9 | catalytic activity | 0.43 |
7. | Q1HRH7 | Catalase (Fragment) (similar to Aedes aegypti) | 20 | 8 | 48.5 | catalytic activity | 0.39 |
8. | Q380N3 | AGAP001797-PA (similar to Anopheles gambiae) | 17 | 17 | 84.3 | Unknown | 0.44 |
9. | Q7Q3D8 | AGAP007827-PA (similar to Anopheles gambiae) | 15 | 10 | 46.6 | phosphopyruvate hydratase activity | 0.068 |
10. | Q7QA89 | AGAP004366-PA (similar to Anopheles gambiae) | 11 | 8 | 63.5 | oxidoreductase activity | 0.60 |
11. | Q7Q5J5 | AGAP006398-PA (similar to Anopheles gambiae) | 10.34 | 3 | 31.2 | unknown function | 0.56 |
12. | F5HKV6 | Fructose-bisphosphate aldolase (similar to Anopheles gambiae) | 9.64 | 4 | 39.2 | aldolase activity | 0.66 |
13. | Q7QDY3 | AGAP010479-PA (similar to Anopheles gambiae) | 7.86 | 4 | 45.0 | unknown | 0.62 |
14. | Q7PQK5 | AGAP004192-PA (similar to Anopheles gambiae) | 7.13 | 6 | 72.7 | Protein folding | 0.62 |
15. | Q7Q609 | AGAP006099-PA (similar to Anopheles gambiae) | 6.61 | 4 | 45.1 | metal ion binding, catalytic activity | 0.45 |
16. | Q7QHE7 | AGAP011158-PA (Fragment) (similar to Anopheles gambiae) | 6.15 | 3 | 51.5 | pyridoxal phosphate binding | 0.64 |
17. | Q7Q5T1 | AGAP006225-PA (similar to Anopheles gambiae) | 5.14 | 8 | 138.7 | oxidoreductase activity | 0.53 |
18. | T1DPT6 | Malic enzyme (Fragment) (similar to Anopheles aquasalis) | 4.43 | 3 | 79.2 | metal ion binding | 0.45 |
19. | F5HLD4 | AGAP003656-PB (similar to Anopheles gambiae) | 4.12 | 18 | 450.9 | protein binding, Immunoglobulin-like domains | 0.65 |
20. | A0A084VWP1 | AGAP001797-PE-like protein (similar to Anopheles sinensis) | 17 | 6 | 41.657 | tropomyosin | 0.48 |
21. | T1DN92 | Putative voltage-dependent anion-selective channel (similar to An. aquasalis) | 8 | 2 | 38.131 | voltage-gated anion channel activity | 0.57 |
22. | Q17GL0 | AAEL002978-PA (similar to Aedes aegypti) | 10 | 5 | 65.476 | aminopeptidase activity | 0.5 |
23. | A0A023EEY5 | Putative cytochrome c (similar to Aedes albopictus) | 21 | 2 | 16.3 | electron carrier activity | 0.62 |
24. | B0WKF4 | Pyrroline-5-carboxylate dehydrogenase (similar to Culex quinquefasciatus) | 10 | 6 | 77.7 | oxidoreductase activity | 0.56 |
25. | A0A023EGV4 | Putative calmodulin (similar to Aedes albopictus) | 25 | 5 | 19.538 | calcium ion binding | 0.27 |
26. | A0A084VKA2 | Calreticulin (similar to Anopheles sinensis) | 7 | 4 | 59.369 | calcium ion binding | 0.5 |
27. | Q7Q343 | AGAP011476-PA (similar to Anopheles gambiae) | 7 | 5 | 105.89 | amidase activity | 0.45 |
28. | A0A084WD61 | AGAP007990-PA-like protein (similar to Anopheles sinensis) | 4 | 3 | 655.68 | glucuronosyl transferase activity | 0.53 |
29. | A0A023EJ61 | Putative 2-cys thioredoxin peroxidase (similar to Aedes albopictus) | 9 | 2 | 267.66 | peroxidase activity | 0.33 |
30. | Q7QGY7 | AGAP010957-PA, partial (similar to Anopheles gambiae) | 24 | 5 | 199.5 | calcium ion binding | 0.27 |
31. | B0WED4 | Deoxyuridine 5′-triphosphate nucleotidohydrolase(similar to Culex quinquefasciatus) | 21 | 3 | 15.9 | Diphosphatase activity | 0.543 |
32. | Q7QFS4 | AGAP003869-PA (similar to Anopheles gambiae) | 11.29 | 6 | 54 | Aminopeptidase activity | 0.646 |
33. | Q7Q3R0 | AGAP007990-PA (similar to Anopheles gambiae) | 10.22 | 6 | 61.4 | transferase activity | 0.380 |
34. | Q7QEF5 | AGAP000679-PA (similar to Anopheles gambiae) | 9 | 4 | 46.4 | Aminoacylase activity | 0.585 |
35. | A0NFA5 | AGAP007790-PA (similar to Anopheles gambiae) | 7.32 | 4 | 35.5 | Ion transport | 0.291 |
36. | A0NDL8 | AGAP003713-PA (similar to Anopheles gambiae) | 6.84 | 1 | 12.8 | Unknown | 0.316 |
37. | Q16V79 | AAEL009658-PB (similar to Aedes aegypti) | 6.84 | 4 | 66.9 | Alpha trehalase activity/ catalytic activity | 0.606 |
38. | B0W447 | Carbonic anhydrase (similar to Culex quinquefasciatus) | 5.76 | 2 | 31.5 | carbonate dehydratase activity | 0.450 |
39. | Q7QF10 | AGAP000249-PA (similar to Anopheles gambiae) | 4.30 | 4 | 69.7 | mannosyl-glycoprotein endo-beta-N-acetyl glucosaminidase activity | 0.520 |
40. | Q7PV84 | AGAP012302-PA (similar to Anopheles gambiae) | 3.66 | 2 | 71.1 | sulfate transmembrane transporter activity | 0.405 |
41. | Q7QAH7 | AGAP003695-PA (similar to Anopheles gambiae) | 3.48 | 5 | 105.5 | metallopeptidase activity | 0.577 |