Fig. 7

Expression analysis of some of VQs from nematode and fungus. a Expression profiling of VQs from 5 nematode species. The RNA-Seq data were achieved from https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2812/. b Expression profiling of the VQ gene F23H12.2 in C. elegans among 17 different developmental stages of tissues. c The genomic DNA sample was isolated from the fungus Gloeophyllum trabeum and was used as the template to isolate the gene with the NCBI accession number XM_007869238.1 by PCR amplification. The used primer sequences were listed in Additional file 1: Table S1. M, marker (1 Kb Ladder); 1, PCR with no template (negative control); 2, with the fungus genomic DNA as template. d Expression analysis of the gene with the NCBI accession number XM_007869238.1 by RT-PCR. M, marker (1 Kb Ladder); 1, RT-PCR with no template (negative control); 2 and 3, with the fungus total RNA as template using Actin primers (positive control) and XM_007869238.1 primers, respectively. e Expression profiling of the VQ gene XM_007869238.1 from Gloeophyllum trabeum. The RNA-Seq data in (b) and (e) were achieved from the NCBI GEO Datasets with accession numbers GSE41367 and GSE108189, respectively. The expression abundance was shown by FPKM (Fragments Per Kilobase Million) values in (a), (b) and (e)