Table 1 Specific qRT-PCR primers used in this study
From: Decreased testosterone levels after caponization leads to abdominal fat deposition in chickens
Gene name | Sequence | Product size (bp) | Accession NO. |
|---|---|---|---|
RXRG | F:5’-AGGCGTGGGCTCCATCTTT-3’ | 259 | NM_205294 |
R:5’-CCGTAGTGCTGGCAGGC-3’ | |||
FADS2 | F:5’-CTGAGGAAGACAGCAGAGGACAT-3’ | 153 | NM_0011604282 |
R:5’-GCAGGCAAGGATTAGAGTTGTG-3’ | |||
FABP7 | F:5’-CGTGATCAGGACTCAGAGCA-3’ | 158 | NM_205308 |
R:5’-TCTCTTTGCCATCCCATTTC-3’ | |||
SCD | F:5’-GGCTGACAAAGTGGTGATG-3’ | 137 | NM_204890 |
R:5’-GGATGGCTGGAATGAAGA-3’ | |||
FABP3 | F:5’-AGTACATGAAGGCGTTGGGG-3’ | 171 | NM_001030889 |
R:5’-CCGTGGTCTCATCGAACTCC-3’ | |||
APOA1 | F:5’-GTGACCCTCGCTGTGCTCTT-3’ | 217 | NM_205525 |
R:5’-CACTCAGCGTGTCCAGGTTGT-3’ | |||
MSMO1 | F:5’-TTCGTACTTCTTGCTCTGCGT-3’ | 226 | NM_001006438 |
R:5’-CCACCTAGGCATCTCTTCCC-3’ | |||
SQLE | F:5’-CGCTGACGGTTGTAGCTGAT-3’ | 200 | NM_001194927 |
R:5’-AAGGACGCGAGTCTCAGTTG-3’ | |||
MGLL | F:5’-GGAGTCCTCAAAACATCCCATAC-3’ | 189 | NM_001277142 |
R:5’-CAAACAAGTTAAGTTCTGTCAGCCT-3’ | |||
PTGDS | F:5’-GACGGACAAGTGTATGGCAG-3’ | 118 | NM_204259 |
R:5’-CCAGGAAGTAAAGGTGGATATGG-3’ | |||
CYP2D6 | F:5’-GATTGGAAGAAACCGACCGC-3’ | 170 | NM_001195557 |
R:5’-TTGTCGTCCCTTTGGGAATG-3’ | |||
β-actin | F:5’-GAGAAATTGTGCGTGACATCA-3’ | 152 | NM_205518 |
R:5’-CCTGAACCTCTCATTGCCA-3’ |