Fig. 1

Transcriptome heterogeneity between oocytes and surrounding cumulus cells. (a) Depiction of the experimental design and the sequenced samples. (b) Principal component analysis of 12,482 genes quantified in oocytes (red), innerCCs (green) and outerCCs (blue). (c) Overlap of genes expressed in oocytes (red), innerCCs (green) or outerCCs (blue). (d) Differentially expressed genes determined from the pairwise comparisons of the three sample types (FDR < 0.01). (e) Co-expression analysis of genes expressed in oocytes showing differential expression compared those in cumulus cells (CC). The blue heatmaps depict the average expression in oocytes and CCs; the center heatmap depicts the topological overlap of correlated expression; and the vertical bars show the annotation of GO biological processes (BP) and molecular functions (MF) enriched in the corresponding gene cluster. The squares below the heatmap provide a link between cluster number, the corresponding biological process or molecular functions and genes presented in the supplementary (Additional file 3: Tables S8-S9)