Fig. 4

Quantitative assessment of the radial distribution of v2r-related OlfC-expressing neurons. The distribution of radial positions of OlfC-expressing cells was quantified for seven OlfC genes, including OlfCc1, using the same set of sections, for which laminar height was determined, except the very first sections, where the sensory surface does not yet extend toward the median raphe. Radial position within the section was normalized to maximal radius, i.e. length of the lamella containing the respective labeled cell. In other words, radial distance was measured from the apex of the lamellar ‘curve’, i.e. closest to the median raphe, to the cell soma center, and normalized to the distance between this apex position (most central) and the border of the epithelial section (most peripheral). a The resulting distributions of relative radius (from 0, innermost to 1, outermost) are shown binned (histogram, top row) and unbinned (ECDF, bottom row). The color code for the OlfC genes is the same as in Fig. 3 to facilitate comparisons between different positional parameters. Light grey curves in the ECDF plot for each of the tested OlfC genes represent the distribution for individual olfactory organs. Due to the smaller number of cells, scatter is increased. b Overlay of the seven distributions shown individually in panel a), both as histogram (left panel) and ECDF (right panel)