Fig. 4

Classification of KOG-identified DEGs into KOG categories under acute heat stress. KOG-identified DEGs from the (a-c) three contrasts (2, 4, and 8 h) between acute heat stress and control conditions (30 °C vs. 20 °C) (see Fig. 1a-c) were classified into the 25 superordinated KOG categories (right). Gene enrichment analyses (chi-square tests) identified KOG categories with deviating regulation, for which the category-specific ratio between upregulated (+) and downregulated (−) DEGs was significantly different from the contrast-specific ratio (^***P ≤ 0.001, black bars; ^**P ≤ 0.01, dark-gray bars; ^*P ≤ 0.05, light-gray bars). (a-c) The KOG categories O, E, and G exhibited highly significant and persistent deviations in regulation under acute heat stress